钙黄绿素 AM 是一种膜渗透性荧光酯酶底物，通常用于检测细胞活性和质膜完整性。作为一种近中性的底物，钙黄绿素 AM 可以自由、无创地扩散穿过质膜并渗透到活细胞的胞质溶胶中。一旦进入细胞内，代谢活跃细胞中的胞质酯酶会迅速将非荧光钙黄绿素 AM 底物水解成钙黄绿素产物，并由具有完整质膜的细胞保留。由于死细胞缺乏酯酶活性，因此仅标记活细胞。在蓝光激发下，钙黄绿素发出强烈的绿色荧光，与细胞酯酶的活性和样品中存在的活细胞数量成正比。与其他活细胞标记试剂（如 BCECF AM 或羧基荧光素二乙酸酯）相比，钙黄绿素 AM 荧光更亮、光稳定性更好，低细胞毒性有利于细胞追踪、细胞凋亡和细胞毒性的研究。
AAT Bioquest 提供多种可供选择的用于细胞活力分析的钙黄绿素 AM 产品。除了传统的绿色荧光钙黄绿素 AM 之外，我们还提供发出蓝色、橙色、红色和深红色荧光的钙黄绿素 AM 衍生物。
钙黄绿素活性染料选择指南
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Dye |
Calcein Blue |
CytoCalcein™ Violet 450 |
Calcein UltraBlue™ |
CytoCalcein™ Violet 500 |
Calcein UltraGreen™ |
Calcein |
Calcein Orange™ |
Calcein Red™ |
Calcein Deep Red™ |
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|
Principle |
In live cells, non-fluorescent calcein AM is converted to a fluorescent calcein product after AM ester hydrolysis by intracellular esterases. |
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Readout |
Live cells are highly fluorescent and easily distinguishable from dead cells, which are non-fluorescent. |
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Laser(nm) |
UV (350) |
Violet (405) |
UV (350) |
Violet (405) |
Blue (488) |
Blue (488) |
Green (532) |
Yellow (561-568) |
Red (633-647) |
|
|
Ex/Em(nm) |
354/441 |
406/445 |
359/458 |
420/505 |
492/514 |
501/521 |
531/545 |
562/576 |
643/663 |
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|
Multiplexable |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
|
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Dye Compatibility |
Compatible with green and red fluorescent proteins, indicators, and other probes |
Compatible with green and red fluorescent proteins, indicators, and other probes |
Compatible with green and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and green fluorescent proteins, indicators, and other probes |
Compatible with blue and green fluorescent proteins, indicators, and other probes |
|
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Sample Type |
Live Cells |
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Platform |
Fluorescence Microscope, Fluorescence Microplate Reader, Flow Cytometer |
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Cat No. |
AM Ester |
西安百萤生物科技有限公司 商家主页地 址： 西安市高新区草堂科技产业基地秦岭大道西6号科技企业加速器二区9幢2单元20401号房 联系人： 第五经理 电 话： 029-68064558 传 真： 029-68064558 Email：info@tjbiolite.com;zx@tjbiolite.com 相关咨询小麦胚芽凝集素（WGA）染料：揭示细胞内秘密的关键利器 (暂无发布时间 浏览数:1797) 百萤问号之Cy3酪胺的实验方案是怎样的 (暂无发布时间 浏览数:4212) AAT Bioquest:低丰度靶标染色神器----TSA和PSA (2024-11-12T00:00 浏览数:4970) 百萤问答之酪胺染料问题及建议 (暂无发布时间 浏览数:4509) 百萤课堂-酪胺 (2024-11-05T00:00 浏览数:4977) 百萤-pH荧光探针Protonex 绿 500, SE产品资料 (2024-10-21T00:00 浏览数:6613) 百萤-pH荧光探针Protonex 红600及pH荧光探针Protonex 红600, SE产品资料 (2024-10-15T00:00 浏览数:9513) Protonex pH荧光染料：解锁细胞世界的酸碱秘密！ (2024-10-08T00:00 浏览数:9264) 西安百萤生物中秋放假通知 (2024-09-13T00:00 浏览数:14016) 百萤课堂之活性氧Reactive Oxygen Species(ROS) (2024-08-28T00:00 浏览数:17485) ADVERTISEMENT
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