分子量:17.6 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Cystatin F, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <7.0 nM.

产品特性:Human Cystatin F, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 17.6 kDa. The predicted N-terminus is Arg 20. The reducing (R) protein migrates as 20-24 kDa in SDS-PAGE  due to glycosylation.

产品背景:Cystatin-7 (CST7) is also known as Cystatin-F, Cystatin-like metastasis-associated protein (CMAP), Leukocystatin, which is a disulfide-linked homodimer belongs to the cystatin family. CST7 is primarily expressed in peripheral blood cells and spleen. CST7 inhibits papain and cathepsin L but with affinities lower than other cystatins. CST7 may play a role in immune regulation through inhibition of a unique target in the hematopoietic system.