UBA3 (C-term) Peptide
产品名称： UBA3 (C-term) Peptide
英文名称： UBA3 (C-term) Peptide
产品编号： P0179
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBA3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBA3.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
Bohnsack RN, Haas AL.J Biol Chem. 2003 Jul 18;278(29):26823-30. Epub 2003 May 10.
- 2.
- Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA.Nature. 2003 Mar 20;422(6929):330-4.
- 3.
- Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
Gong L, Yeh ET.J Biol Chem. 1999 Apr 23;274(17):12036-42.
- Applications
- Western Blot (Tissue lysate)
- The UBA3 polyclonal antibody (Cat # PAB1774) is used in Western blot to detect UBA3 in mouse brain tissue lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with UBA3 polyclonal antibody (Cat # PAB1774) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 9039
- Protein Accession#:
- NP_003959;Q8TBC4
- Gene Name:
- UBA3
- Gene Alias:
- DKFZp566J164,MGC22384,UBE1C,hUBA3
- Gene Description:
- ubiquitin-like modifier activating enzyme 3
- Omim ID:
- 603172
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- Nedd8-activating enzyme hUba3,UBA3, ubiquitin-activating enzyme E1 homolog,ubiquitin-activating enzyme 3,ubiquitin-activating enzyme E1C (UBA3 homolog, yeast),ubiquitin-activating enzyme E1C (homologous to yeast UBA3)
- Gene Pathway