• 经测试应用WB, IHC-P, ICC/IFmore details
• 种属反应性
  与反应: Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 53BP2/ASPP2 aa 50-150. The exact sequence is proprietary.
  Database link: Q13625

• 阳性对照
  • Jurkat, Saos-2, MCF7 cell lysate; Human kidney tissue; Human testis tissue
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.2
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 0.05% BSA, 50% Glycerol
• 纯度Tissue culture supernatant
• 克隆单克隆
• 克隆编号EPR13837
• 同种型IgG
• 研究领域
  • Cell Biology
   
  • Cell Cycle
   
  • Cell Cycle Inhibitors
   
  • p53

应用

• 应用说明Is unsuitable for Flow Cyt or IP.

靶标

• 功能Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
• 组织特异性Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
• 序列相似性Belongs to the ASPP family.
  Contains 2 ANK repeats.
  Contains 1 SH3 domain.
• 结构域The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
• 细胞定位Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.

• Anti-53BP2/ASPP2 antibody [EPR13837] 图像

  • 

    Western blot - Anti-53BP2/ASPP2 [EPR13837] antibody (ab181377)
    All lanes : Anti-53BP2/ASPP2 antibody [EPR13837] (ab181377) at 1/10000 dilution
    
    Lane 1 : Jurkat cell lysate
    Lane 2 : Saos-2 cell lysate
    Lane 3 : MCF7 cell lysate
    
    Lysates/proteins at 10 µg per lane.
    
    
    Predicted band size : 126 kDa
    
  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2 [EPR13837] antibody (ab181377)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling 53BP2/ASPP2 with ab181377 at a 1/100 dilution.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2 [EPR13837] antibody (ab181377)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling 53BP2/ASPP2 with ab181377 at a 1/100 dilution.

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2 [EPR13837] antibody (ab181377)

    Immunofluorescent analysis of Jurkat cells labeling 53BP2/ASPP2 with ab181377 at a 1/50 dilution.