分子量:31.8 kDa (FCGRT), 13.1 kDa (B2M) 

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Rat FcRn / FCGRT & B2M by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA. 
Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Rat FcRn / FCGRT & B2M(Cat# FCM-R5287 ) with a linear range of 0.06-2.5 μg/mL.

产品特性:Rat FcRn / FCGRT & B2M Heterodimer Protein, produced by co-expression of FcRN and B2M, has a calculated MW of 31.8 kDa (FCGRT / FcRN) and 13.1 kDa (B2M). Subunit FCGRT / FcRN is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The predicted N-terminus is Ala 23 (FcRN) & Ile 21 (B2M). The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14.5 kDa (B2M) respectively due to different glycosylation.

产品背景:FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.