PML polyclonal antibody
产品名称： PML polyclonal antibody
英文名称： PML polyclonal antibody
产品编号： PAB4342
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PML.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to human PML.
- Sequence:
- HQWFLKHEARPLAELR
- Host:
- Rabbit
- Reactivity:
- Human
- Specificity:
- This antibody recognize synthetic peptide (HQWFLKHEARPLAELR) containing a predicted sumoylation site of human PML.
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML.
Favre M, Butticaz C, Stevenson B, Jongeneel CV, Telenti A.J Acquir Immune Defic Syndr. 2003 Oct 1;34(2):127-33.
- 2.
- Leukemia-associated translocation products able to activate RAS modify PML and render cells sensitive to arsenic-induced apoptosis.
Puccetti E, Beissert T, Guller S, Li JE, Hoelzer D, Ottmann OG, Ruthardt M.Oncogene. 2003 Oct 9;22(44):6900-8.
- 3.
- ZIP kinase triggers apoptosis from nuclear PML oncogenic domains.
Kawai T, Akira S, Reed JC.Mol Cell Biol. 2003 Sep;23(17):6174-86.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PML polyclonal antibody (Cat # PAB4342) in K-562 cell line lysates . Nonsumoylated PML (arrow) was detected using the purified PML polyclonal antibody (Cat # PAB4342) .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PML polyclonal antibody (Cat # PAB4342) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Entrez GeneID:
- 5371
- Protein Accession#:
- NP_150242;P29590
- Gene Name:
- PML
- Gene Alias:
- MYL,PP8675,RNF71,TRIM19
- Gene Description:
- promyelocytic leukemia
- Omim ID:
- 102578
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
- Other Designations:
- promyelocytic leukemia protein,promyelocytic leukemia, inducer of,tripartite motif protein TRIM19