CHEK1 (phospho S280) Peptide
产品名称： CHEK1 (phospho S280) Peptide
英文名称： CHEK1 (phospho S280) Peptide
产品编号： P1300
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK1.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S280of human CHEK1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase.
Kramer A, Mailand N, Lukas C, Syljuasen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J.Nat Cell Biol. 2004 Sep;6(9):884-91. Epub 2004 Aug 15.
- 2.
- Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
Xu X, Lee J, Stern DF.J Biol Chem. 2004 Aug 13;279(33):34091-4. Epub 2004 Jun 25.
- 3.
- Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains.
Ng CP, Lee HC, Ho CW, Arooz T, Siu WY, Lau A, Poon RY.J Biol Chem. 2004 Mar 5;279(10):8808-19. Epub 2003 Dec 16.
- Applications
- Western Blot (Tissue lysate)
- The CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) is used in Western blot to detect Phospho-CHK1-S280 in mouse brain tissue lysate.
- Western Blot (Cell lysate)
- The CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, Ramos, Jurkat, HL-60, and Hela cell lysates.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Gene Pathway
- Related Disease
- Adenocarcinoma
- Adenofibroma
- Ataxia telangiectasia
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Pancreatic Ductal
- Colon cancer
- Colonic Neoplasms
- Colorectal Neoplasms
- Colorectal Neoplasms, Hereditary Nonpolyposis
- DNA Damage
- Esophageal Neoplasms
- Genetic Predisposition to Disease
- Glioma
- Kidney Failure, Chronic
- Lung Neoplasms
- Meningeal Neoplasms