FriOI内切酶-RNAi技术-试剂-生物在线
上海沪震实业有限公司
FriOI内切酶

FriOI内切酶

商家询价

产品名称: FriOI内切酶

英文名称: FriOI

产品编号: HZ2138

产品价格: 0

产品产地: 中国/上海

品牌商标: 沪震生物

更新时间: 2023-08-17T10:24:20

使用范围: null

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 FriOI内切酶

中文名称:FriOI内切酶
英文名称:FriOI
产品规格:500U
储存条件:冷冻(-20℃)

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格
 

Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites GRGCY↑C
来源 Flavobacterium rigense O
Assayed on Lambda DNA
Optimal buffer Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+?BSA
Optimal temperature 37℃
Storage conditions 10 mM Tris-HCl(pH 7.5);200 mM KCl;0.1 mM EDTA;200 μg/ml BSA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃.
Ligations After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a.of enzyme for 16hoursat 37℃.
Methlation effect Methylation sensitivity not tested
Thermal inactivation enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends BanII,Eco24I,EcoT38I,HgiJII

 

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