内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Immobilized Human BMP-2, Tag Free (Catalog # BM2-H4117) at 2μg/mL (100 μL/well) can bind Human Gremlin, His Tag (Catalog # GR1-H52H3) with a linear range of 0.8-81.9 ng/mL.

产品背景:
Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.

产品描述:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-35 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.